This product is prepared by labeling high quality goat anti-mouse IgG (H+L) with the green fluorescent dye CF488A. CF488A is a superior alternative to the traditional green fluorescent dye FITC (or fluorescein) owing to its exceptional brightness and photostability. CF488A is comparably bright and photostable to Alexa Fluor 488. However, antibody conjugates prepared from CF488A are more specific than those prepared from Alexa Fluor 488 because CF488A, being minimally charged, does not significantly change the isoelectric points of antibodies as Alexa Fluor 488 does (Please download CF488A dye flyer for more details).
|Catelog Number||Unit Size|
Images and Data:
HeLa cells were stained with mouse anti-golgin 97 and rabbit anti-COX IV primary antibodies followed by CF488A goat anti-mouse and CF555 goat anti-rabbit secondary antibodies. CF647-phalloidin was used to label F-actin and Hoechst 33342 was used to stain for DNA. (Green=golgi, red=mitochondria, blue=nucleus, and magenta=cytoskeleton).
Absorption and emission spectra of CF488A, Alexa Fluor 488 and fluorescein on goat anti-mouse IgG in pH 7.4 PBS buffer.