Equipamentos para Laboratórios | Equipamento para Laboratório

The InviMag Plant DNA Mini Kit/ KF96 allows for rapid and economical purification of genomic DNA from plant samples using magnetic beads. The InviMag Plant DNA Mini Kit/ KF96 has been designed for semi-automated preparation on the KingFisher 96 and Flex 96 workstations. Up to 96 samples can be processed in parallel.

Starting Material

fresh, frozen or dried plant material (like seed leaves, roots, fruits etc.), food of plant origin

Product Characteristics

Advantages

• ultra-pure, ready to use genomic DNA for enhanced performance in sensitive downstream applications
• rapid isolation of ready-to-use DNA, free of polysaccharides and other secondary metabolites
• reproducible yields
• no cross contaminations
• minimal hands-on-time

Overview

To realize an efficient lysis and highest yields, the plant samples are first mechanically disrupted followed by lysis in an optimized buffer and Proteinase K. Cell debris is removed by centrifugation. The cleared lysates are transferred to the subsequent automatic purification procedure. After the direct lysis step the DNA binds to the magnetic beads and RNA can now be removed by RNase digestion. During washing steps the DNA is bound on the beads while contaminants, like enzyme inhibitors remain in the wash buffer, allfollowed by the final elution. The procedures are designed to avoid sample-to-sample cross contamination.
The purified high quality DNA is ready to use for subsequent downstream applications or can be stored at –20 °C for subsequent use:

• PCR
• genotyping (e.g. detection of GMOs in various materials like soy and corn)
• restriction digestion

Ordering Information