Equipamentos para Laboratórios | Equipamento para Laboratório

The InviMag Virus DNA Mini Kit/ KFmL allows for rapid and economical purification of viral DNA from up to 200 µl of clinical samples* using magnetic beads. The InviMag Virus DNA Mini Kit/ KFmL has been designed for semi-automated preparation on the KingFisher mL workstation. The amount of purified viral DNA in the kit procedure depends on the sample type, the virus titer, sample source, transport, storage, and age.

Starting Material

fresh or frozen plasma, serum, whole blood, cell-free body fluids of human origin, rinse liquid from swabs

Product Characteristics

Advantages

• highly efficient recovery of high-quality viral DNA from different sources, allowing for very sensitive virus detection
• reproducible yields
• linear yields - efficient purification over a wide range of viral titers
• Carrier RNA is provided in the kit
• highly suitable for in vitro diagnostics (CE-marked for compliance with EU Directive 98/79/EC in Europe)*

Overview

Aside from the pre-filling of all cavities, all processing steps are performed by the robot. The sample is lysed in an optimized lysis buffer containing Proteinase K and Carrier RNA. The addition of Carrier RNA is necessary for the enhancement of DNA recovery in samples with low virus load. Carrier RNA also stabilizes nucleic acids in samples with very small nucleic acid concentrations. The lysis step can be carried out directly on the platform at ambient temperature or outside on a thermomixer while continuously shaking. In this case the lysed samples are transferred onto the workstation to the subsequent automatic purification procedure based on magnetic beads. The viral DNA binds to the magnetic beads, followed by washing steps and the final elution. Manual handling of potentially infectious samples is minimized, ensuring safety to the user and reliable processing of samples.
Due to the high purity, the isolated viral DNA is ready to use for a broad panel of downstream applications:

• PCR
• real-time PCR (quantitative PCR, like TaqMan and LightCycler technologies)
• array technologies

*) USA: for research use only - not for use in diagnostic procedures

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