Equipamentos para Laboratórios | Equipamento para Laboratório

Catalog #	Size	Concentration	Qty
M0250L	7,500 units	10,000 units/ml	1
M0250S	1,500 units	10,000 units/ml	1

Download: MSDS PDF

• Removal of single-stranded extensions (3'and 5') to leave ligatable blunt ends
• Transcriptional mapping
• Cleavage of hairpin loops
• Supplied with 10X Reaction Buffer

Description:

A single-strand specific DNA and RNA endonuclease which will degrade single-stranded extensions from the ends of DNA and RNA molecules, leaving blunt, ligatable ends.

Source:

Mung bean sprouts

Applications:

• Removal of 3´ and 5´ extensions from DNA or RNA termini
• Transcriptional mapping
• Cleavage of hairpin loops
• Excision of gene coding sequences from genomic DNA
• Generation of new restriction sites

Reagents Supplied:
Mung Bean Nuclease Reaction Buffer (10X)

Enzyme Properties

Molecular Weight:
Theoretical: 39 kDa

Reaction & Storage Conditions

Reaction Conditions:
1X Mung Bean Nuclease Reaction Buffer
Incubate at 30°C.

1X Mung Bean Nuclease Reaction Buffer:
50 mM sodium acetate
30 mM NaCl
1 mM ZnSO₄
pH 5.0 @ 25°C

Unit Definition:
One unit is defined as the amount of enzyme required to produce 1 µg of acid-soluble total nucleotide in a total reaction volume of 50 μl in 1 minute at 37°C in 1X Mung Bean Nuclease Reaction Buffer with 0.5 mg/ml denatured Calf Thymus DNA.

Concentration:
10,000 units/ml

Storage Conditions:
10 mM sodium acetate
0.1 mM zinc acetate
1 mM Cysteine
50% Glycerol
0.001% Triton X-100
pH 5.0 @ 25°C

Storage Temperature:
-20°C

Notes

Usage notes:

1. It is no longer necessary to supplement Mung Bean Nuclease reactions with Zn²⁺. The zinc acetate in the storage buffer fulfills the Zn²⁺ requirement of the enzyme even after dilution in a reaction.

FAQs

1. Should I use Mung Bean Nuclease or DNA Polymerase I, Large (Klenow) Fragment (NEB# M0210)?
2. Is the Mung Bean Nuclease active in other NEBuffers?
3. Can Mung Bean Nuclease be heat inactivated?
4. Does Zn²⁺ need to be added to a Mung Bean Nuclease reaction?
5. Will Mung Bean Nuclease degrade double stranded DNA?

Protocols

Protocols for Mung Bean Nuclease

Quality Control for Current Lot

Quality control values for a specific lot can be found on the datacard which accompanies each product.

Quality Assurance Statement:
Purified free of double-strand exonuclease contamination.

Terminal Integrity:
After incubation of 1 μg of lambda DNA (Hae III digest) with 10 units of Mung Bean Nuclease at 30ºC for 30 hour(s), > 95% of the DNA fragments can be ligated with T4 DNA ligase. Of these ligated fragments, >95% can be recut with Hae III.

A) Molecular weight standard (λ HindIII digest)
B) M13mp8 (supercoiled)
C) As in B after incubation with NcoI
D) As in B after incubation with HindIII
E) As in D after incubation with Mung Bean Nuclease
F) As in E after incubation with T4 DNA Ligase
G) As in F after incubation with NcoI
H) As in F after incubation with HindIII

References

1. Kowalski, D. et al. (1976) Biochemistry, 15, 4457-4463.
2. McCutchan, T.F. et al. (1984) Science, 225, 626-628.

Reagents Sold Separately

Mung Bean Nuclease Reaction Buffer