LumiteinTM protein gel stain is a luminescent dye designed for detecting total proteins in polyacrylamide gels following gel electrophoresis. The stain combines excellent sensitivity, exceptional user-friendliness and compatibility.
LumiteinTM protein gel stain is supplied as a highly concentrated 100X solution, which provides convenience for shipping, handling and storage. More importantly, the concentrated solution is economical because of reduced manufacturing and shipping costs.
|Catelog Number||Unit Size|
Highly Sensitive: At least as sensitive as silver stain by detecting as little as 1 ng or less protein; detecting more spots than SYPRO Ruby in 2-D gel staining.
Extremely Simple & Fast Staining: Fixation and staining is a single combined step. Use the 30-min Rapid Protocol for excellent result, or the 90-min Basic Protocol for the ultimate sensitivity in 1-D gel staining; no overstaining with longer staining time.
Excellent Compatibility with Existing Instruments: Can be used with either a simple UV-box (designed for DNA gel viewing), a Dark Reader, or a high-end laser scanner.
Wide Linear Detection Range: At least three orders of magnitude.
Perfectly Compatible with Downstream Analysis: Compatible with MS and sequencing.
Economical: Supplied as a 100X concentrated solution to reduce manufacturing cost and shipping cost, resulting in significant saving for customers.
Stable: Both the 100X concentrated solution and the 1X working solution are stable at room temperature for at least 1 year.
Figure 1. Two-fold serial dilutions of Precision Plus protein standard (Bio-Rad) were separated via SDS-PAGE and then stained with LumiteinTM according to manufacturer-recommended protocol. Images were taken with GE Typhoon Trio using 532 nm excitation and 610BP30 emission filter.
Figure 2. 2-D gel of human liver protein lysate stained with LumiteinTM.The three circled spots were picked for MS analysis by Applied Biomics, Inc. (Hayward, CA). The result confirmed that LumiteinTM staining is fully compatible with MS analysis (data not shown).